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Chapter 3 Tools of Microbiology

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  • Simple stains contain
    one dye.
  • Using a small amount of specimen for growth is
    inoculation.
  • Media that is comprised of unknown composition is
    complex.
  • Magnification with a light microscopy is done with the ocular and objective
    lenses.
  • A light background on a slide is due to a ____ microscope.
    bright-field
  • Microbial food is
    media (medium).
  • Using a glass "hockey stick" to distribute microbes on a plate is the
    spread plate method.
  • Endospore, capsule and flagellar are examples of
    special stains.
  • A microscope that can provide an internal image of a cell and its organelles is a
    transmission electron microscope.
  • The growth of microorganisms under ideal conditions is
    incubation.
  • The complete rupture of red blood cells is measured as
    beta-hemolysis.
  • Oil immersion prevents light
    refraction.
  • Media that allows one species to grow but prohibits the growth of others is
    selective.
  • Hanging drop mounts allow specimens to
    move.
  • _____ is used to make media solid.
    Agar
  • Specimen collection must be done using
    sterile technique.
  • Media that allows all species to grow but they appear uniquely is
    differential.
  • The cultivation or growth of organisms is
    culturing.
  • Broths, milks, and infusions are examples of
    liquid media.
  • Transferring dilute amounts onto plates to isolate colonies is the
    loop dilution method.
  • An illuminated specimen with a dark background is due to a ________ microscope
    dark-field
  • Media where all components are known is
    chemically defined (synthetic).
  • A discrete mound of one species of cell on a solid surface is a
    colony.
  • The three components of microscopy are
    magnification, resolution and contrast.
  • Isolation requires _____ to form individual colonies.
    solid media
  • A _____ microscope can focus along several planes.
    confocal
  • Gram and acid-fast are examples of
    differential stains.
  • Pure cultures can also be called
    anexic.
  • Phenotypic, genotypic and immunologic tests are all part of the ____ step.
    identification
  • A microscope that creates a 3-D image of the specimen is a
    scanning electron microscope.
  • The incomplete rupture of red blood cells is
    alpha-hemolysis.
  • The method of inoculating in quadrants to thin the quantity of microbes is the
    streak plate method.
  • Contrast is achieved for light microscopy due to
    staining.
  • To measure motility, you must use
    semisolid media.
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